Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Deubiquitylase YOD1 regulates CDK1 stability and drives triple-negative breast cancer tumorigenesis

doi: 10.1186/s13046-023-02781-3

Figure Lengend Snippet: Correlation of YOD1 expression with clinical features of patients with breast cancer

Article Snippet: NTNBC cell lines (MCF10A, MCF7, T47D and ZR75-1), TNBC cell lines (MDA-MB-468, MDA-MB-231 and BT549), and a human embryonic kidney cell line (HEK293T) were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Biomarker Discovery

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Deubiquitylase YOD1 regulates CDK1 stability and drives triple-negative breast cancer tumorigenesis

doi: 10.1186/s13046-023-02781-3

Figure Lengend Snippet: YOD1 deubiquitylates CDK1 and maintains CDK1 stability. A , Western blotting showed the expression of CDK1 in NTNBC cells (MCF10A, MCF7, T47D, ZR75-1) and TNBC cells (MDA-MB-231, MDA-MB-468, BT-549). B-C , Evaluate the expression of CDK1 when YOD1 gain and loss of function were performed in MDA-MB-231 and BT-549 cells by western blotting. D , Immunoprecipitation combined with western blotting showed the CDK1 ubiquitination levels in NTNBC and TNBC cells. E-F , Evaluate CDK1 ubiquitination levels when YOD1 gain and loss of function were performed in the indicated cells. G , Flag-tagged YOD1 and its truncated constructs were transfected respectively into 293T cells to evaluate expression of CDK1. The cell lysates were subjected to immunoprecipitation with anti-CDK1 antibodies to detect CDK1 ubiquitination levels. H , His-tagged UB and Flag-YOD1 constructs were co-transfected into 293T cells to evaluate expression of CDK1 and exogenous constructs expression. The cell lysates were subjected to immunoprecipitation with anti-CDK1 and detected exogenous ubiquitination levels

Article Snippet: NTNBC cell lines (MCF10A, MCF7, T47D and ZR75-1), TNBC cell lines (MDA-MB-468, MDA-MB-231 and BT549), and a human embryonic kidney cell line (HEK293T) were obtained from the American Type Culture Collection (ATCC).

Techniques: Western Blot, Expressing, Immunoprecipitation, Ubiquitin Proteomics, Construct, Transfection